Purpose(Empty Backbone) Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41391||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 9308
Vector typeMammalian Expression, Lentiviral ; Gateway Destination vector
- Promoter PGK
/ Fusion Protein
- V5 (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsccdB resistant bacteria. 30C for liquid culture. 37C for plates.
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer hPGK-F (GTGTTCCGCATTCTGCAAG)
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Plasmid for constitutive lentiviral expression.
There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence.
Alternate plasmid names:
SV40-puro; PGK-gateway-V5 tag
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLEX_306 was a gift from David Root (Addgene plasmid # 41391 ; http://n2t.net/addgene:41391 ; RRID:Addgene_41391)