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Purpose(Empty Backbone) Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLKO
- Backbone size (bp) 9260
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Vector typeMammalian Expression, Lentiviral ; Gateway Destination vector
- Promoter PGK
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdB resistant bacteria. 30C for liquid culture. 37C for plates.
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer hPGK-F (GTGTTCCGCATTCTGCAAG)
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid for constitutive lentiviral expression.
Alternate plasmid names:
pLX305,
SV40-puro; PGK-gateway-no tag
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX_305 was a gift from David Root (Addgene plasmid # 41390 ; http://n2t.net/addgene:41390 ; RRID:Addgene_41390)
