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A genetically encoded photoactivatable Rac controls the motility of living cells.
Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM
Nature. 2009 Sep 3. 461(7260):104-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
22007pTriEx-mVenus-PA-Rac1Expression of photoactivatable Rac1
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22008pBabe-TetCMV-puro-mVenus-PA-Rac1Mammalian expression of photoactivatable Rac1
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22017pTriEx-mVenus-PA-Rac1-T17NExpression of photoactivatable Rac1 (dominant-negative mutant)
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22018pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N
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22021pTriEx-mVenus-PA-Rac1-C450AExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
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22022pTriEx-mPA-GFP-PA-Rac1Expression of photoactivatable Rac1
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22023pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
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22024pTriEx-PA-Rac1Expression of photoactivatable Rac1
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22025pTriEx-PA-Rac1-C450AExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
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22026pTriEx-PA-Rac1-I539EExpression of photoactivatable Rac1 ('lit' state mutant)
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22027pTriEx-mCherry-PA-Rac1Expression of photoactivatable Rac1
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22028pTriEx-mCherry-PA-Rac1-C450AExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
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22029pTriEx-mCherry-PA-Rac1-T17NExpression of photoactivatable Rac1 (dominant-negative mutant).
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22030pTriEx-mCerulean-PA-Rac1Expression of photoactivatable Rac1
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22031pBabe-TetCMV-puro-mCerulean-PA-Rac1
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22032pBabe-TetCMV-puro-mPA-GFP-PA-Rac1
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22033pBabe-TetCMV-puro-mVenus-LOVMammalian expression of mVenus tagged LOV2 domain
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22034pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M
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22035pBabe-TetCMV-puro-mCherry-PA-Rac1Expression of photoactivatable Rac1
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75263PA-Cdc42Light-activated Cdc42
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Antibodies from Article