|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19909||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5512
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDamage-specific DNA binding protein 1
SpeciesH. sapiens (human)
Insert Size (bp)3420
Mutationplease see depositor comment below
Entrez GeneDDB1 (a.k.a. DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site -- (not destroyed)
- 3′ cloning site -- (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Please note that Addgene's quality control result found mutations I253T, I258L and M679V in DDB1 when compared to the Genbank NM_001923. The plasmid should function as described in the original publication (Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA2-DDB1 was a gift from Yue Xiong (Addgene plasmid # 19909 ; http://n2t.net/addgene:19909 ; RRID:Addgene_19909)
For your References section:WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y. Genes Dev. 2008 Apr 1. 22(7):866-71. 10.1101/gad.1624008 PubMed 18381890