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pcDNA3-HA2-DDB1
(Plasmid #19909)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19909 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-HA2
  • Backbone size w/o insert (bp) 5512
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Damage-specific DNA binding protein 1
  • Alt name
    DDB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3420
  • Mutation
    please see depositor comment below
  • GenBank ID
    NM_001923
  • Entrez Gene
    DDB1 (a.k.a. DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site -- (not destroyed)
  • 3′ cloning site -- (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note that Addgene's quality control result found mutations I253T, I258L and M679V in DDB1 when compared to the Genbank NM_001923. The plasmid should function as described in the original publication (Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA2-DDB1 was a gift from Yue Xiong (Addgene plasmid # 19909 ; http://n2t.net/addgene:19909 ; RRID:Addgene_19909)
  • For your References section:

    WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y. Genes Dev. 2008 Apr 1. 22(7):866-71. 10.1101/gad.1624008 PubMed 18381890