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(Plasmid #21538)


Item Catalog # Description Quantity Price (USD)
Plasmid 21538 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4752
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    The first 7aa of this Ago2 protein are different from the NCBI website Ref sequ
  • GenBank ID
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original Ago2 clone from Dr. Tom Hobman at Univ. of Calgary
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The first 7aa of our Ago2 protein are different from NCBI website Ref seq. Addgene NGS results identified an R372M variant in Ago2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Ago2 was a gift from Edward Chan (Addgene plasmid # 21538 ; ; RRID:Addgene_21538)
  • For your References section:

    The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK. RNA. 2009 May . 15(5):804-13. 10.1261/rna.1229409 PubMed 19324964