|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21538||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4752
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)2600
MutationThe first 7aa of this Ago2 protein are different from the NCBI website Ref sequ
Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
/ Fusion Protein
- 3XFLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV24sp (Common Sequencing Primers)
The first 7aa of our Ago2 protein are different from NCBI website Ref seq. Addgene NGS results identified an R372M variant in Ago2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLAG-Ago2 was a gift from Edward Chan (Addgene plasmid # 21538 ; http://n2t.net/addgene:21538 ; RRID:Addgene_21538)
For your References section:The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK. RNA. 2009 May . 15(5):804-13. 10.1261/rna.1229409 PubMed 19324964