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A piggyBac-based toolkit for inducible genome editing in mammalian cells.
Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM
RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
126028PB_rtTA_BsmBIPiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning
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126029PB_tre_Cas9PiggyBac cargo vector expressing doxycycline inducible Cas9
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126030PB_tre_dCas9_KRABPiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion
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126031PB_tre_dCas9_VP160PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion
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Antibodies from Article