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(Plasmid #126029)


Item Catalog # Description Quantity Price (USD)
Plasmid 126029 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    piggyBac cargo vector
  • Backbone manufacturer
    System Biosciences
  • Total vector size (bp) 11497
  • Vector type
    Mammalian Expression, CRISPR ; piggyBac
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
    humanized S. pyrogenes Cas9
  • Insert Size (bp)
  • Promoter TRE

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Hygromycin resistance
  • Insert Size (bp)
  • Promoter EF1 alpha

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cas9 from pX330 (Addgene#42230; Cong et al., 2013)
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB_tre_Cas9 was a gift from Mauro Calabrese (Addgene plasmid # 126029 ; ; RRID:Addgene_126029)
  • For your References section:

    A piggyBac-based toolkit for inducible genome editing in mammalian cells. Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. 10.1261/rna.068932.118 PubMed 31101683