PB_tre_Cas9
(Plasmid #126029)

• Purpose: PiggyBac cargo vector expressing doxycycline inducible Cas9
• Depositing Lab: Mauro Calabrese
• Publication: Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118.

Backbone

• Vector backbone: piggyBac cargo vector
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 11497
• Vector type: Mammalian Expression, CRISPR ; piggyBac
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: humanized S. pyrogenes Cas9
• Insert Size (bp): 4272
• Promoter: TRE

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGGAAAAGGCCTCCACGG

Gene/Insert 2

• Gene/Insert name: Hygromycin resistance
• Insert Size (bp): 1008
• Promoter: EF1 alpha

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • PB_tre_Cas9.ape
• A portion of this plasmid was derived from a plasmid made by: Cas9 from pX330 (Addgene#42230; Cong et al., 2013)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/448803v1 for BioRxiv preprint