pB-rtTA
(Plasmid #126034)

• Purpose: PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.
• Depositing Lab: Mauro Calabrese
• Publication: Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. doi: 10.1038/s41588-018-0207-8. Epub 2018 Sep 17.

Backbone

• Vector backbone: piggyBac cargo vector
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 8306
• Vector type: Mammalian Expression ; piggyBac
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
• Species: Synthetic
• Insert Size (bp): 3846
• Promoter: hUbiC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • rtTA_cargo.ape
• A portion of this plasmid was derived from a plasmid made by: The hUbiC-rtTA3-IRES-Neo cassette was cloned from pSLIK-Neo (Addgene#25735)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry