Purpose(Empty Backbone) piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||200030||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6000
Modifications to backboneGene inserts with U6 promoter and GFP (EF1a promoter, WPRE, and SV40-polyA element).
Vector typeMammalian Expression
- Promoter U6, EF1a
/ Fusion Protein
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:piggyBAC-U6-GFP-WPRE was a gift from Jay Shendure (Addgene plasmid # 200030 ; http://n2t.net/addgene:200030 ; RRID:Addgene_200030)