Multiplexed DNA repair assays for multiple lesions and multiple doses via transcription inhibition and transcriptional mutagenesis.
Nagel ZD, Margulies CM, Chaim IA, McRee SK, Mazzucato P, Ahmad A, Abo RP, Butty VL, Forget AL, Samson LD
Proc Natl Acad Sci U S A. 2014 May 6;111(18):E1823-32. doi: 10.1073/pnas.1401182111. Epub 2014 Apr 22. PubMed Article
Nagel ZD, Margulies CM, Chaim IA, McRee SK, Mazzucato P, Ahmad A, Abo RP, Butty VL, Forget AL, Samson LD
Proc Natl Acad Sci U S A. 2014 May 6;111(18):E1823-32. doi: 10.1073/pnas.1401182111. Epub 2014 Apr 22. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177823 | pMax_BFP_A191G | Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR). |
| 177824 | pMax_ScaI_BFP | Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR). |
| 177825 | pMax_GFP | Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone) |
| 177826 | pMax_GFP_C289T | Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR). |
| 177827 | pMax_mOrange | Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone) |
| 177828 | pMax_mOrange_A215C | Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR). |
| 177829 | pMax_mOrange_G299C | Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR). |
| 177830 | pMax_mPlum | Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone) |
| 177831 | pMax_mPlum_C207G/T208C | Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR). |