Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells.
Weinberg BH, Pham NT, Caraballo LD, Lozanoski T, Engel A, Bhatia S, Wong WW
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87549pBW1997_pExpr-hU6-CRISPR-DECODERExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases
Loading...
87551pBW842_pCAG-pExpr-FP-DECODERExpresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases
Loading...
87552pBW820_pCAG-pExpr-FULL-ADDEREncodes full adder logic in response to Cre, Flp and VCre recombinases
Loading...
87554pBW829_pCAG-pExpr-LUTEncodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases
Loading...
87559pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpAExpresses aa1-396 of Flp recombinase fused to ABI CID
Loading...
87560pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpAExpresses aa397-423 of Flp recombinase fused to PYL CID
Loading...
87561pBW701_pCAG-PV1Simple PV1
Loading...
87562pBW702_pCAG-PV2Simple PV2
Loading...
87563pBW703_pCAG-PV3Simple PV3
Loading...
87564pBW704_pCAG-PV4Simple PV4
Loading...
87565pBW751_pCAG-PV5Simple PV5
Loading...
87566pBW752_pCAG-PV6Simple PV6
Loading...
87567pBW753_pCAG-PV7Simple PV7
Loading...
87568pBW754_pCAG-PV8Simple PV8
Loading...
87569pBW705_pCAG-PV1-FRT-lox2272-STOPPV1 for 2-input, 2-output circuit library
Loading...
87570pBW706_pCAG-PV2-lox2272-GFPPV2 for 2-input, 2-output circuit library
Loading...
87571pBW707_pCAG-PV3-FRT-loxP-mCherryPV3 for 2-input, 2-output circuit library
Loading...
87572pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNAPV4 gRNA entry vector for CRISPR decoder
Loading...
87574pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpAPV1 for 2-input, 4-output decoder circuit
Loading...
87575pBW759_pCAG-PV2-lox2272-GFP-BGHpAPV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder
Loading...
87576pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpAPV3 for 2-input, 4-output decoder circuit
Loading...
87577pBW770_pCAG-PV4-loxP-mRuby2-BGHpAPV4 for 2-input, 4-output decoder circuit
Loading...
87578pBW700_pCAG-pDest-U1-UXpCAG destination vector (U1-UX)
Loading...
87579pBW1716_hU6-pDest-U1-UXhU6 destination vector
Loading...
87580pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpAPV1 for 3-input, 2-output Full Adder
Loading...
87582pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpAPV3 for 3-input, 2-output Full Adder
Loading...
87583pBW807_pCAG-PV4-loxP-mCherry-BGHpAPV4 for 3-input, 2-output Full Adder
Loading...
87584pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpAPV5 for 3-input, 2-output Full Adder
Loading...
87585pBW809_pCAG-PV6-F3-mCherry-BGHpAPV6 for 3-input, 2-output Full Adder
Loading...
87586pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpAPV7 for 3-input, 2-output Full Adder
Loading...
87587pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpAPV8 for 3-input, 2-output Full Adder
Loading...
89053pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNAPV1 gRNA entry vector for CRISPR decoder
Loading...
89055pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNAPV2 gRNA entry vector for CRISPR decoder
Loading...
89056pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNAPV3 gRNA entry vector for CRISPR decoder
Loading...
89057pBW708_pCAG-PV4-loxP-GFP-T2A-mCherryPV4 for 2-input, 2-output circuit library
Loading...
89507pBW755_pCAG-pDest-U5-UXpCAG destination vector (U5-UX)
Loading...
89573pCAG-iCreExpresses iCre under pCAG
Loading...
89574pCAG-FlpOExpresses FlpO under pCAG
Loading...
89575pCAG-VCreExpresses VCre under pCAG
Loading...
89684pCAG-EGFPpCAG promoter driving EGFP
Loading...
89685pCAG-mtagBFPpCAG promoter driving mtagBFP
Loading...
89686pCAG-mRuby2pCAG promoter driving mRuby2
Loading...
89687pCAG-iRFP720pCAG promoter driving iRFP720
Loading...
89688pCAG-LSS-mOrangepCAG promoter driving LSS-mOrange
Loading...
89689pCAG-FALSEpCAG promoter driving no CDS
Loading...