Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells.
Weinberg BH, Pham NT, Caraballo LD, Lozanoski T, Engel A, Bhatia S, Wong WW
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805. PubMed Article
Weinberg BH, Pham NT, Caraballo LD, Lozanoski T, Engel A, Bhatia S, Wong WW
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
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| 87549 | pBW1997_pExpr-hU6-CRISPR-DECODER | Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases |
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| 87551 | pBW842_pCAG-pExpr-FP-DECODER | Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases |
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| 87552 | pBW820_pCAG-pExpr-FULL-ADDER | Encodes full adder logic in response to Cre, Flp and VCre recombinases |
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| 87554 | pBW829_pCAG-pExpr-LUT | Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases |
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| 87559 | pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA | Expresses aa1-396 of Flp recombinase fused to ABI CID |
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| 87560 | pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA | Expresses aa397-423 of Flp recombinase fused to PYL CID |
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| 87561 | pBW701_pCAG-PV1 | Simple PV1 |
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| 87562 | pBW702_pCAG-PV2 | Simple PV2 |
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| 87563 | pBW703_pCAG-PV3 | Simple PV3 |
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| 87564 | pBW704_pCAG-PV4 | Simple PV4 |
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| 87565 | pBW751_pCAG-PV5 | Simple PV5 |
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| 87566 | pBW752_pCAG-PV6 | Simple PV6 |
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| 87567 | pBW753_pCAG-PV7 | Simple PV7 |
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| 87568 | pBW754_pCAG-PV8 | Simple PV8 |
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| 87569 | pBW705_pCAG-PV1-FRT-lox2272-STOP | PV1 for 2-input, 2-output circuit library |
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| 87570 | pBW706_pCAG-PV2-lox2272-GFP | PV2 for 2-input, 2-output circuit library |
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| 87571 | pBW707_pCAG-PV3-FRT-loxP-mCherry | PV3 for 2-input, 2-output circuit library |
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| 87572 | pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA | PV4 gRNA entry vector for CRISPR decoder |
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| 87574 | pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA | PV1 for 2-input, 4-output decoder circuit |
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| 87575 | pBW759_pCAG-PV2-lox2272-GFP-BGHpA | PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder |
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| 87576 | pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA | PV3 for 2-input, 4-output decoder circuit |
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| 87577 | pBW770_pCAG-PV4-loxP-mRuby2-BGHpA | PV4 for 2-input, 4-output decoder circuit |
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| 87578 | pBW700_pCAG-pDest-U1-UX | pCAG destination vector (U1-UX) |
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| 87579 | pBW1716_hU6-pDest-U1-UX | hU6 destination vector |
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| 87580 | pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA | PV1 for 3-input, 2-output Full Adder |
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| 87582 | pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA | PV3 for 3-input, 2-output Full Adder |
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| 87583 | pBW807_pCAG-PV4-loxP-mCherry-BGHpA | PV4 for 3-input, 2-output Full Adder |
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| 87584 | pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA | PV5 for 3-input, 2-output Full Adder |
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| 87585 | pBW809_pCAG-PV6-F3-mCherry-BGHpA | PV6 for 3-input, 2-output Full Adder |
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| 87586 | pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA | PV7 for 3-input, 2-output Full Adder |
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| 87587 | pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA | PV8 for 3-input, 2-output Full Adder |
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| 89053 | pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA | PV1 gRNA entry vector for CRISPR decoder |
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| 89055 | pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA | PV2 gRNA entry vector for CRISPR decoder |
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| 89056 | pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA | PV3 gRNA entry vector for CRISPR decoder |
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| 89057 | pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry | PV4 for 2-input, 2-output circuit library |
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| 89507 | pBW755_pCAG-pDest-U5-UX | pCAG destination vector (U5-UX) |
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| 89573 | pCAG-iCre | Expresses iCre under pCAG |
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| 89574 | pCAG-FlpO | Expresses FlpO under pCAG |
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| 89575 | pCAG-VCre | Expresses VCre under pCAG |
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| 89684 | pCAG-EGFP | pCAG promoter driving EGFP |
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| 89685 | pCAG-mtagBFP | pCAG promoter driving mtagBFP |
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| 89686 | pCAG-mRuby2 | pCAG promoter driving mRuby2 |
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| 89687 | pCAG-iRFP720 | pCAG promoter driving iRFP720 |
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| 89688 | pCAG-LSS-mOrange | pCAG promoter driving LSS-mOrange |
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| 89689 | pCAG-FALSE | pCAG promoter driving no CDS |
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