PLJR965
(Plasmid #115163)

• Purpose: Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis
• Depositing Lab: Sarah Fortune
• Publication: Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi: 10.1038/nmicrobiol.2016.274.

Backbone

• Vector backbone: custom design
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Sth1 sgRNA scaffold
• Species: Streptcoccus thermophilus
• Insert Size (bp): 101

Gene/Insert 2

• Gene/Insert name: Sth1 dCas9
• Alt name: nuclease-dead dcas9 allele
• Species: Streptcoccus thermophilus
• Insert Size (bp): 3366

Gene/Insert 3

• Gene/Insert name: TetR (deleted TetON) MtbCO
• Alt name: Tet repressor protein
• Insert Size (bp): 624

Gene/Insert 4

• Gene/Insert name: L5 attP
• Insert Size (bp): 43

Gene/Insert 5

• Gene/Insert name: L5 Int
• Insert Size (bp): 1116

Gene/Insert 6

• Gene/Insert name: KanR
• Alt name: Kanamycin resistance protein
• Insert Size (bp): 816

Resource Information

• Supplemental Documents:
  • PLJR965 (Mtb CRISPRi).gbk
  • CRISPRi protocol for Msmeg and Mtb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The group constructed this plasmid in a BSL1 laboratory for the purpose of expressing them in BSL2 organism (such as M. smegmatis) or BSL3 (such as M. tuberculosis). Otherwise, if it's only in E. coli, then it is BSL1.