Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #115163)


Item Catalog # Description Quantity Price (USD)
Plasmid 115163 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    custom design
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Sth1 sgRNA scaffold
  • Species
    Streptcoccus thermophilus
  • Insert Size (bp)

Gene/Insert 2

  • Gene/Insert name
    Sth1 dCas9
  • Alt name
    nuclease-dead dcas9 allele
  • Species
    Streptcoccus thermophilus
  • Insert Size (bp)

Gene/Insert 3

  • Gene/Insert name
    TetR (deleted TetON) MtbCO
  • Alt name
    Tet repressor protein
  • Insert Size (bp)

Gene/Insert 4

  • Gene/Insert name
    L5 attP
  • Insert Size (bp)

Gene/Insert 5

  • Gene/Insert name
    L5 Int
  • Insert Size (bp)

Gene/Insert 6

  • Gene/Insert name
  • Alt name
    Kanamycin resistance protein
  • Insert Size (bp)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The group constructed this plasmid in a BSL1 laboratory for the purpose of expressing them in BSL2 organism (such as M. smegmatis) or BSL3 (such as M. tuberculosis). Otherwise, if it's only in E. coli, then it is BSL1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLJR965 was a gift from Sarah Fortune (Addgene plasmid # 115163 ; ; RRID:Addgene_115163)
  • For your References section:

    Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Rock JM, Hopkins FF, Chavez A, Diallo M, Chase MR, Gerrick ER, Pritchard JR, Church GM, Rubin EJ, Sassetti CM, Schnappinger D, Fortune SM. Nat Microbiol. 2017 Feb 6;2:16274. doi: 10.1038/nmicrobiol.2016.274. 10.1038/nmicrobiol.2016.274 PubMed 28165460