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pKM464 (deletions)
(Plasmid #108322)


Item Catalog # Description Quantity Price (USD)
Plasmid 108322 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Kenan Murphy
  • Total vector size (bp) 3082
  • Modifications to backbone
    Insertion of Bxb1 attB site
  • Vector type
    ORBIT integration plasmid for creating knockouts of target genes
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Bxb1 attB site
  • Species
  • Insert Size (bp)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORBIT integrating plasmid for creating knockouts of target genes.

Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM464 (deletions) was a gift from Kenan Murphy (Addgene plasmid # 108322 ; ; RRID:Addgene_108322)
  • For your References section:

    ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes. Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM. MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18. 10.1128/mBio.01467-18 PubMed 30538179