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pKM444
(Plasmid #108319)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108319 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUV15tet-O
  • Backbone manufacturer
    Dirk Schnappinger and Sabine Ehrt
  • Modifications to backbone
    Insertion of RecT and Bxb1 Integrase. Removal of GFP and Hyg.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Che9C RecT
  • Species
    mycobacterial phage
  • Insert Size (bp)
    1062

Gene/Insert 2

  • Gene/Insert name
    Bxb1 Integrase
  • Species
    mycobacterial phage
  • Insert Size (bp)
    1503

Gene/Insert 3

  • Gene/Insert name
    Tet Repressor
  • Species
    bacterial
  • Insert Size (bp)
    624

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Julia van Kessel and Graham Hatfull (RecT generated from pJV53); Michele Calos (Addgene plasmid pCMV-Bx was the source for Bxb1 Integrase.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

The plasmid is induced with 500 ng/ml anhydrotetracycline to induce expression of RecT and Bxb1 Integrase for performing the ORTBIT protocol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM444 was a gift from Kenan Murphy (Addgene plasmid # 108319 ; http://n2t.net/addgene:108319 ; RRID:Addgene_108319)
  • For your References section:

    ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes. Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM. MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18. 10.1128/mBio.01467-18 PubMed 30538179