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pKM468-EGFP
(Plasmid #108434)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108434 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKM446
  • Backbone manufacturer
    Kenan Murphy
  • Backbone size w/o insert (bp) 3159
  • Total vector size (bp) 3879
  • Modifications to backbone
    Insertion of attB-EGFP sequence
  • Vector type
    ORBIT integrating plasmid
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow in LB containing 20 ug/ml chloramphenicol.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    attB-eGFP-4xGly-TEV-Flag-6xHis
  • Species
    Synthetic
  • Insert Size (bp)
    829

Resource Information

Depositor Comments

ORBIT plasmid for creating EGFP fusions to target genes.

Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM468-EGFP was a gift from Kenan Murphy (Addgene plasmid # 108434 ; http://n2t.net/addgene:108434 ; RRID:Addgene_108434)
  • For your References section:

    ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes. Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM. MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18. 10.1128/mBio.01467-18 PubMed 30538179