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PLJR962
(Plasmid #115162)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115162 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Sth1 sgRNA scaffold
  • Species
    Streptcoccus thermophilus
  • Insert Size (bp)
    101

Gene/Insert 2

  • Gene/Insert name
    Sth1 dCas9
  • Alt name
    nuclease-dead dcas9 allele
  • Species
    Streptcoccus thermophilus
  • Insert Size (bp)
    3366

Gene/Insert 3

  • Gene/Insert name
    TetR
  • Alt name
    Tet repressor protein
  • Insert Size (bp)
    624

Gene/Insert 4

  • Gene/Insert name
    L5 attP
  • Insert Size (bp)
    43

Gene/Insert 5

  • Gene/Insert name
    L5 Int
  • Insert Size (bp)
    1116

Gene/Insert 6

  • Gene/Insert name
    KanR
  • Alt name
    Kanamycin resistance protein
  • Insert Size (bp)
    816

Resource Information

Depositor Comments

The group constructed this plasmid in a BSL1 laboratory for the purpose of expressing them in BSL2 organism (such as M. smegmatis) or BSL3 (such as M. tuberculosis). Otherwise, if it's only in E. coli, then it is BSL1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLJR962 was a gift from Sarah Fortune (Addgene plasmid # 115162 ; http://n2t.net/addgene:115162 ; RRID:Addgene_115162)
  • For your References section:

    Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Rock JM, Hopkins FF, Chavez A, Diallo M, Chase MR, Gerrick ER, Pritchard JR, Church GM, Rubin EJ, Sassetti CM, Schnappinger D, Fortune SM. Nat Microbiol. 2017 Feb 6;2:16274. doi: 10.1038/nmicrobiol.2016.274. 10.1038/nmicrobiol.2016.274 PubMed 28165460