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(Plasmid #140993)


Item Catalog # Description Quantity Price (USD)
Plasmid 140993 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    custom design
  • Total vector size (bp) 8881
  • Vector type
    Unspecified ; integrative in mycobactreia

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    type II CRISPR RNA-guided endonuclease Cas9
  • Alt name
    Streptococcus thermophilus LMD-9
  • Species
    Streptococcus thermophilus
  • Insert Size (bp)
  • GenBank ID

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Insert Size (bp)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    L5 attP
  • Insert Size (bp)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    L5 Int
  • Insert Size (bp)

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
  • Insert Size (bp)

Cloning Information for Gene/Insert 5

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sarah Fortune and Jeremy Rock

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPRx-Sth1Cas9-L5 was a gift from Wilbert Bitter (Addgene plasmid # 140993 ; ; RRID:Addgene_140993)
  • For your References section:

    Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9. Meijers AS, Troost R, Ummels R, Maaskant J, Speer A, Nejentsev S, Bitter W, Kuijl CP. Tuberculosis (Edinb). 2020 Sep;124:101983. doi: 10.1016/ Epub 2020 Aug 12. 10.1016/ PubMed 32829077