Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCRISPRx-Sth1Cas9-L5
(Plasmid #140993)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140993 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PLJR962
  • Backbone manufacturer
    custom design
  • Total vector size (bp) 8881
  • Vector type
    Unspecified ; integrative in mycobactreia

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Sth1Cas9
  • Alt name
    type II CRISPR RNA-guided endonuclease Cas9
  • Alt name
    Streptococcus thermophilus LMD-9
  • Species
    Streptococcus thermophilus
  • Insert Size (bp)
    3366
  • GenBank ID

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    TetR
  • Insert Size (bp)
    624

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    L5 attP
  • Insert Size (bp)
    43

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    L5 Int
  • Insert Size (bp)
    1116

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    KanR
  • Insert Size (bp)
    816

Cloning Information for Gene/Insert 5

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sarah Fortune and Jeremy Rock
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPRx-Sth1Cas9-L5 was a gift from Wilbert Bitter (Addgene plasmid # 140993 ; http://n2t.net/addgene:140993 ; RRID:Addgene_140993)
  • For your References section:

    Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9. Meijers AS, Troost R, Ummels R, Maaskant J, Speer A, Nejentsev S, Bitter W, Kuijl CP. Tuberculosis (2020) 101983 10.1016/j.tube.2020.101983