Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


HA PKB T308D S473D pcDNA3
(Plasmid #14751)


Item Catalog # Description Quantity Price (USD)
Plasmid 14751 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PKB DD
  • Alt name
    Protein Kinase B alpha
  • Alt name
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
  • Mutation
    T308D S473D. Constitutively active mutant.
  • Entrez Gene
    AKT1 (a.k.a. BOS_20453)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KpnI(1934) and NheI(2429) represent T308->D and S473->D mutations respectively

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA PKB T308D S473D pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14751 ; ; RRID:Addgene_14751)
  • For your References section:

    Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Scheid MP, Marignani PA, Woodgett JR. Mol Cell Biol. 2002 Sep . 22(17):6247-60. 10.1128/MCB.22.17.6247-6260.2002 PubMed 12167717