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PurposeMammalian expression of human GSK3 beta constitutively active mutant with HA tag
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Depositing Lab
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Depositing OrganizationSinai Health System
Located in Canada -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 14754-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 14754-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK3 beta S9A
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Alt nameGSK3b
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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MutationS9A. Constitutively active mutant.
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Entrez GeneGSK3B
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S2A in the sequence does not affect function, used to introduce restriction enzyme site
DNA (Catalog # 14754-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 14754-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA GSK3 beta S9A pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14754 ; http://n2t.net/addgene:14754 ; RRID:Addgene_14754) -
For your References section:
Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Stambolic V, Woodgett JR. Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):701-4. doi: 10.1042/bj3030701. 10.1042/bj3030701 PubMed 7980435
Map uploaded by the depositor.
