GSK3 beta pGEX
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15898||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameGSK3 beta
SpeciesH. sapiens (human)
Insert Size (bp)1400
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
ORF is at 258-2237.
The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid chanage. The plasmid encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GSK3 beta pGEX was a gift from Jim Woodgett (Addgene plasmid # 15898 ; http://n2t.net/addgene:15898 ; RRID:Addgene_15898)