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HA GSK3 beta K85A pcDNA3
(Plasmid #14755)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14755 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK3 beta K85A
  • Alt name
    GSK3b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    K85A. Kinase dead mutant.
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Nru I site is added to the K->A mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA GSK3 beta K85A pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14755 ; http://n2t.net/addgene:14755 ; RRID:Addgene_14755)