pDEST/LIfeAct-mCherry-N1
(Plasmid #40908)

• Depositing Lab: Robin Shaw
• Publication: Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epub 2012 Feb 9.

Backbone

• Vector backbone: pmcherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 4773
• Modifications to backbone: Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LifeAct
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 51
• Mutation: No stop codon to create c-terminal fusion
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: mCherry Rev

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry