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pDEST/LIfeAct-mCherry-N1
(Plasmid #40908)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40908 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmcherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 4773
  • Modifications to backbone
    Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    51
  • Mutation
    No stop codon to create c-terminal fusion
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST/LIfeAct-mCherry-N1 was a gift from Robin Shaw (Addgene plasmid # 40908 ; http://n2t.net/addgene:40908 ; RRID:Addgene_40908)
  • For your References section:

    Actin cytoskeleton rest stops regulate anterograde traffic of connexin 43 vesicles to the plasma membrane. Smyth JW, Vogan JM, Buch PJ, Zhang SS, Fong TS, Hong TT, Shaw RM. Circ Res. 2012 Mar 30;110(7):978-89. Epub 2012 Feb 9. 10.1161/CIRCRESAHA.111.257964 PubMed 22328533