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pmTurquoise2-Mito
(Plasmid #36208)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36208 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmTurquoise2-C1
  • Total vector size (bp) 4823
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTS
  • Alt name
    Mitochondrial Targeting Signal
  • Alt name
    COX8A(1-29)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    89
  • GenBank ID
    NM_004074
  • Promoter CMV
  • Tag / Fusion Protein
    • mTurquoise2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer AGGTCTATATAAGCAGAGC
  • 3′ sequencing primer TCTACAAATGTGGTATGGC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene's quality control sequence with EGFP-C primer shows that there is a single base change downstream of the mTurquoise in the SV40 terminator that eliminates the MluI site. Upstream of the mTurquoise, our sequence with EGFP-N primer shows a small insertion adding several additional restriction sites. These changes are in the cloning/junction region and should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmTurquoise2-Mito was a gift from Dorus Gadella (Addgene plasmid # 36208 ; http://n2t.net/addgene:36208 ; RRID:Addgene_36208)
  • For your References section:

    Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A. Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. 10.1038/ncomms1738 PubMed 22434194