|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47608||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerClontech; modified as described in Rogers et al JBC 2001
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt namemyosin X
SpeciesH. sapiens (human)
Insert Size (bp)6167
MutationSee depositor comments below.
Entrez GeneMYO10 (a.k.a. MyoX)
- Promoter CMV
/ Fusion Protein
- EGFP-HA-His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EGFPC1-hMyoX was a gift from Emanuel Strehler (Addgene plasmid # 47608 ; http://n2t.net/addgene:47608 ; RRID:Addgene_47608)
For your References section:Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. Bennett RD, Mauer AS, Strehler EE. J Biol Chem. 2007 Feb 2;282(5):3205-12. Epub 2006 Nov 26. 10.1074/jbc.M607174200 PubMed 17130134