EGFPC1-hMyoX
(Plasmid #47608)

• Depositing Lab: Emanuel Strehler
• Publication: Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12. Epub 2006 Nov 26.

Backbone

• Vector backbone: EGFPC1
• Backbone manufacturer: Clontech; modified as described in Rogers et al JBC 2001
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MyoX
• Alt name: MYO10
• Alt name: myosin X
• Species: H. sapiens (human)
• Insert Size (bp): 6167
• Mutation: See depositor comments below.
• Entrez Gene: MYO10 (a.k.a. MyoX)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP-HA-His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication.