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EGFPC1-hMyoX
(Plasmid #47608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47608 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 47608-D50 50 μg of DNA in Tris buffer $495
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DNA 47608-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    EGFPC1
  • Backbone manufacturer
    Clontech; modified as described in Rogers et al JBC 2001
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoX
  • Alt name
    MYO10
  • Alt name
    myosin X
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6167
  • Mutation
    See depositor comments below.
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP-HA-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFPC1-hMyoX was a gift from Emanuel Strehler (Addgene plasmid # 47608 ; http://n2t.net/addgene:47608 ; RRID:Addgene_47608)

  • For your References section:

    Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. Bennett RD, Mauer AS, Strehler EE. J Biol Chem. 2007 Feb 2;282(5):3205-12. Epub 2006 Nov 26. 10.1074/jbc.M607174200 PubMed 17130134
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