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pEFmEGFP-mDia2
(Plasmid #25407)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 25407 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EF-BOS
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mDia2
  • Alt name
    drf3
  • Alt name
    diap3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3500
  • Mutation
    Encodes mDia2
  • GenBank ID
    AF094519
  • Entrez Gene
    Diaph3 (a.k.a. 4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Myc (N terminal on insert)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, BglII (destroyed during cloning)
  • 3′ cloning site XbaI, SpeI (destroyed during cloning)
  • 5′ sequencing primer EF1a_fwd
  • 3′ sequencing primer CCA GGG CAT TAG CCA CAC CAG CCA CCA CTT TCT G
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFmEGFP-mDia2 was a gift from Arthur Alberts (Addgene plasmid # 25407 ; http://n2t.net/addgene:25407 ; RRID:Addgene_25407)
  • For your References section:

    Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase signaling. Tominaga T, Sahai E, Chardin P, McCormick F, Courtneidge SA, Alberts AS. Mol Cell. 2000 Jan . 5(1):13-25. 10.1016/S1097-2765(00)80399-8 PubMed 10678165