pEFmEGFP-mDia2
(Plasmid #25407)

• Depositing Lab: Arthur Alberts
• Publication: Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Backbone

• Vector backbone: EF-BOS
• Backbone manufacturer: N/A
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mDia2
• Alt name: drf3
• Alt name: diap3
• Species: M. musculus (mouse)
• Insert Size (bp): 3500
• Mutation: Encodes mDia2
• GenBank ID: AF094519
• Entrez Gene: Diaph3 (a.k.a. 4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2)
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Myc (N terminal on insert)
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI, BglII (destroyed during cloning)
• 3′ cloning site: XbaI, SpeI (destroyed during cloning)
• 5′ sequencing primer: EF1a_fwd
• 3′ sequencing primer: CCA GGG CAT TAG CCA CAC CAG CCA CCA CTT TCT G

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry