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pEFmEGFP-mDia2
(Plasmid #25407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25407 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 25407-D50 50 μg of DNA in Tris buffer $495
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DNA 25407-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    EF-BOS
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mDia2
  • Alt name
    drf3
  • Alt name
    diap3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3500
  • Mutation
    Encodes mDia2
  • GenBank ID
    AF094519
  • Entrez Gene
    Diaph3 (a.k.a. 4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Myc (N terminal on insert)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, BglII (destroyed during cloning)
  • 3′ cloning site XbaI, SpeI (destroyed during cloning)
  • 5′ sequencing primer EF1a_fwd
  • 3′ sequencing primer CCA GGG CAT TAG CCA CAC CAG CCA CCA CTT TCT G
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFmEGFP-mDia2 was a gift from Arthur Alberts (Addgene plasmid # 25407 ; http://n2t.net/addgene:25407 ; RRID:Addgene_25407)

  • For your References section:

    Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase signaling. Tominaga T, Sahai E, Chardin P, McCormick F, Courtneidge SA, Alberts AS. Mol Cell. 2000 Jan . 5(1):13-25. 10.1016/S1097-2765(00)80399-8 PubMed 10678165
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