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GFP-CA-mDia1
(Plasmid #45583)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45583 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Constitutively active mDia1 (CA-mDia1)
  • Alt name
    mDia1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3818
  • Mutation
    Insert mDia1 contains mutations V161D and N165D in the N-terminus, and mutations M1182A and L1185A at the C-terminus
  • Entrez Gene
    Diaph1 (a.k.a. D18Wsu154e, Dia1, Diap1, Drf1, p140mDia)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-CA-mDia1 was a gift from Klaus Hahn & Ronen Zaidel-Bar (Addgene plasmid # 45583 ; http://n2t.net/addgene:45583 ; RRID:Addgene_45583)
  • For your References section:

    An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Rao MV, Chu PH, Hahn KM, Zaidel-Bar R. Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24. 10.1002/cm.21115 PubMed 23677607