GFP-CA-mDia1
(Plasmid #45583)

• Depositing Labs: Klaus Hahn, Ronen Zaidel-Bar
• Publication: Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 8500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Constitutively active mDia1 (CA-mDia1)
• Alt name: mDia1
• Species: M. musculus (mouse)
• Insert Size (bp): 3818
• Mutation: Insert mDia1 contains mutations V161D and N165D in the N-terminus, and mutations M1182A and L1185A at the C-terminus
• Entrez Gene: Diaph1 (a.k.a. D18Wsu154e, Dia1, Diap1, Drf1, p140mDia)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry