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(Plasmid #25419)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 25419 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    clontech Invitrogen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    mDia1 FH2∆N2 sequence (encodes amino acids 771-1182)
  • GenBank ID
  • Entrez Gene
    Diaph1 (a.k.a. D18Wsu154e, Dia1, Diap1, Drf1, p140mDia)
  • Tag / Fusion Protein
    • YFP A206K (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Narumiya Lab Kyoto University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also see Copeland, J. W., and R. Treisman. 2002. The Diaphanous-related Formin mDia1 Controls Serum Response Factor Activity through its Effects on Actin Polymerization. Mol Biol Cell 13:4088-99 for information about FH2∆N2 mutation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-mDia1FH2deltaN was a gift from Arthur Alberts (Addgene plasmid # 25419 ; ; RRID:Addgene_25419)
  • For your References section:

    RhoB and the mammalian Diaphanous-related formin mDia2 in endosome trafficking. Wallar BJ, Deward AD, Resau JH, Alberts AS. Exp Cell Res. 2007 Feb 1. 313(3):560-71. 10.1016/j.yexcr.2006.10.033 PubMed 17198702