|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46825||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDr. Richard Iggo, University of St Andrews, Edinburgh, UK
- Backbone size w/o insert (bp) 9195
- Total vector size (bp) 10380
Modifications to backbonepRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
Alt nameguanine nucleotide binding protein (G protein) alpha 12
SpeciesH. sapiens (human)
Insert Size (bp)1185
MutationQ231L (the mutation reduces GTPase activity resulting in a constitutively active phenotype)
Entrez GeneGNA12 (a.k.a. HG1M1, NNX3, RMP, gep)
- Promoter PGK
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer hPGK-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
cDNA to make this plasmid was obtained from www.cdna.org (ID GNA12000C0).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psd44-G12QL was a gift from Agnese Mariotti (Addgene plasmid # 46825 ; http://n2t.net/addgene:46825 ; RRID:Addgene_46825)