|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46826||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDr. Richard Iggo, University of St Andrews, Edinburgh, UK
Modifications to backbonepRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameg protein alpha q
Alt nameguanine nucleotide binding protein (G protein), q polypeptide
SpeciesH. sapiens (human)
Insert Size (bp)1120
MutationQ209L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)
Entrez GeneGNAQ (a.k.a. CMC1, G-ALPHA-q, GAQ, SWS)
- Promoter PGK promoter
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer hPGK-F (Common Sequencing Primers)
cDNA to make this plasmid was obtained from www.cdna.org (ID GNA0Q000C0).
Addgene NGS identified an IS1 element in the plasmid sequence, consistent with other psd44 plasmids.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psd44-GqQL was a gift from Agnese Mariotti (Addgene plasmid # 46826 ; http://n2t.net/addgene:46826 ; RRID:Addgene_46826)