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(Plasmid #46826)


Item Catalog # Description Quantity Price (USD)
Plasmid 46826 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
  • Modifications to backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    g protein alpha q
  • Alt name
  • Alt name
    guanine nucleotide binding protein (G protein), q polypeptide
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Q209L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)
  • Entrez Gene
    GNAQ (a.k.a. CMAL, CMC1, G-ALPHA-q, GAQ, SWS)
  • Promoter PGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer hPGK-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA to make this plasmid was obtained from (ID GNA0Q000C0).

Addgene NGS identified an IS1 element in the plasmid sequence, consistent with other psd44 plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psd44-GqQL was a gift from Agnese Mariotti (Addgene plasmid # 46826 ; ; RRID:Addgene_46826)