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Addgene

pBiex-1 Myosin VI HMM_eGFP
(Plasmid #82714)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82714 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBiex1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5475
  • Total vector size (bp) 9088
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myosin VI
  • Alt name
    MYO6
  • Species
    Sus scrofa
  • Insert Size (bp)
    3840
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • eGFP (C terminal on insert)
    • FLAG purification tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Foward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiex-1 Myosin VI HMM_eGFP was a gift from Sivaraj Sivaramakrishnan (Addgene plasmid # 82714 ; http://n2t.net/addgene:82714 ; RRID:Addgene_82714)
  • For your References section:

    Patterning Protein Complexes on DNA Nanostructures Using a GFP-Nanobody. Sommese RF, Hariadi RF, Kim K, Liu M, Tyska MJ, Sivaramakrishnan S. Protein Sci. 2016 Aug 18. doi: 10.1002/pro.3020. 10.1002/pro.3020 PubMed 27538185