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Addgene

pBiex-1 Myosin VI HMM_SNAP
(Plasmid #82716)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82716 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBiex1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5475
  • Total vector size (bp) 8950
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myosin VI
  • Alt name
    MYO6
  • Species
    Sus scrofa
  • Insert Size (bp)
    3699
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • SNAP tag (C terminal on insert)
    • FLAG purification tag (C terminal on insert)
    • 6xHis Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Foward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiex-1 Myosin VI HMM_SNAP was a gift from Sivaraj Sivaramakrishnan (Addgene plasmid # 82716 ; http://n2t.net/addgene:82716 ; RRID:Addgene_82716)
  • For your References section:

    Mechanical coordination in motor ensembles revealed using engineered artificial myosin filaments. Hariadi RF, Sommese RF, Adhikari AS, Taylor RE, Sutton S, Spudich JA, Sivaramakrishnan S. Nat Nanotechnol. 2015 Aug;10(8):696-700. doi: 10.1038/nnano.2015.132. Epub 2015 Jul 6. 10.1038/nnano.2015.132 PubMed 26149240