PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53095||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5238
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namemVenus - 3x GS linker - LOV2-Jα (404-546) - 3x GS linker - DAD (1177-1222)
SpeciesM. musculus (mouse); Avena sativa
MutationL47F and C49S in Venus
/ Fusion Proteins
- 6x His (N terminal on backbone)
- mVenus (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pTriExUP
- 3′ sequencing primer pTriExDOWN (Common Sequencing Primers)
Expresses photoactivatable (PA) Diaphanous auto-regulatory domain (DAD) consisting of a fusion of mVenus, LOV2 Jα protein domain of Avena sativa Phototropin1, and Diaphanous auto-regulatory domain of mouse Diaphanous-related formin-1 (mDia1)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PA-DAD-wt was a gift from Klaus Hahn & Ronen Zaidel-Bar (Addgene plasmid # 53095 ; http://n2t.net/addgene:53095 ; RRID:Addgene_53095)
For your References section:An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Rao MV, Chu PH, Hahn KM, Zaidel-Bar R. Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24. 10.1002/cm.21115 PubMed 23677607