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Addgene

pcDNA3.1-6His-MyoX
(Plasmid #47607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47607 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoX
  • Alt name
    MYO10
  • Alt name
    myosin X
  • Species
    H. sapiens (human)
  • GenBank ID
    AF234532.1
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The His tag from the backbone is not in-frame with the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-6His-MyoX was a gift from Emanuel Strehler (Addgene plasmid # 47607 ; http://n2t.net/addgene:47607 ; RRID:Addgene_47607)
  • For your References section:

    Calmodulin-like protein enhances myosin-10 translation. Bennett RD, Strehler EE. Biochem Biophys Res Commun. 2008 May 2;369(2):654-9. doi: 10.1016/j.bbrc.2008.02.056. Epub 2008 Feb 22. 10.1016/j.bbrc.2008.02.056 PubMed 18295593