Purpose(Empty Backbone) dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100091||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Protein
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer poly TK-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:dCas9 plasmid was a gift from David Segal (Addgene plasmid # 100091 ; http://n2t.net/addgene:100091 ; RRID:Addgene_100091)
For your References section:dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression. O'Geen H, Ren C, Nicolet CM, Perez AA, Halmai J, Le VM, Mackay JP, Farnham PJ, Segal DJ. Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578. 10.1093/nar/gkx578 PubMed 28973434