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DNMT3A-dCas9
(Plasmid #100090)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100090 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.3-TOPO
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5407
  • Total vector size (bp) 10864
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNMT3A
  • Species
    H. sapiens (human)
  • Mutation
    includes aa 602-912
  • GenBank ID
    NP_072046.2
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, M.HsaIIIA, TBRS)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFLag-NLS-DNMT3A-dCas9-NLS

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DNMT3A-dCas9 was a gift from David Segal (Addgene plasmid # 100090 ; http://n2t.net/addgene:100090 ; RRID:Addgene_100090)
  • For your References section:

    dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression. O'Geen H, Ren C, Nicolet CM, Perez AA, Halmai J, Le VM, Mackay JP, Farnham PJ, Segal DJ. Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578. 10.1093/nar/gkx578 PubMed 28973434