165,839 results

1. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

   Plasmid

   #234070

   Purpose

   For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-shRNA-ctrl

   Plasmid

   #85741

   Purpose

   shRNA ctrl

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   shRNA ctrl
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available Since

   April 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACAGW-ChR2-Venus-AAV (AAV9)

   Viral Prep

   #20071-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karel Svoboda

   Article

   Leopoldo et al Nature. 2009 Jan 18.

   Promoter

   CAGG

   Tags

   Venus

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

4. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AMPK(T>A)-SPARK

   Plasmid

   #226256

   Purpose

   phospho-null mutant of AMPK-SPARK

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   AMPK(T>A)-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   substituted Threonine 20 with Alanine

   Promoter

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCaggs-mGold2t

   Plasmid

   #231764

   Purpose

   Expression of mGold2t in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mBATF

    Plasmid

    #34575

    Depositor

    Peter Murray

    Article

    Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

    Insert

    BATF (Batf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pKM496 (deletions) Zeo

    Plasmid

    #109301

    Purpose

    ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3puro-EGFP-GAPDH

    Plasmid

    #225475

    Purpose

    Express mEGFP-fusion protein of GAPDH

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG1159 TOM20-ECFP-FIREmate

    Plasmid

    #196693

    Purpose

    Expresses TOM20-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    TOM20-ECFP-FIREmate
    

    Use

    Tags

    FIREmate

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep

    #98747-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Alexander Nectow, Nathaniel Heintz, Eric Schmidt

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Promoter

    EF1a

    Tags

    EGFP (Cre-dependent)

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge