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pCDNA3 Flag Jnk2a2
(Plasmid #13755)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13755 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jnk2a2
  • Alt name
    MAPK9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • GenBank ID
  • Entrez Gene
    MAPK9 (a.k.a. JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag Jnk2a2 was a gift from Roger Davis (Addgene plasmid # 13755 ; http://n2t.net/addgene:13755 ; RRID:Addgene_13755)
  • For your References section:

    Selective interaction of JNK protein kinase isoforms with transcription factors. Gupta S, Barrett T, Whitmarsh AJ, Cavanagh J, Sluss HK, Derijard B, Davis RJ. EMBO J. 1996 Jun 3. 15(11):2760-70. PubMed 8654373