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pCDNA3 Flag Jnk1a1
(Plasmid #13798)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13798 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jnk1a1
  • Alt name
    MAPK8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_002750
  • Entrez Gene
    MAPK8 (a.k.a. JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag Jnk1a1 was a gift from Roger Davis (Addgene plasmid # 13798 ; http://n2t.net/addgene:13798 ; RRID:Addgene_13798)
  • For your References section:

    JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Derijard B, Hibi M, Wu IH, Barrett T, Su B, Deng T, Karin M, Davis RJ. Cell. 1994 Mar 25. 76(6):1025-37. 10.1016/0092-8674(94)90380-8 PubMed 8137421