|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21142||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneCasp1 (a.k.a. ICE, Il1bc)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Sal I (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hgh-pA-R (Common Sequencing Primers)
Compared to GenBank reference sequence BC008152, the caspase-1 insert in this plasmid contains a D56N mutation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-Caspase1-flag was a gift from Junying Yuan (Addgene plasmid # 21142 ; http://n2t.net/addgene:21142 ; RRID:Addgene_21142)
For your References section:Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity. Li J, Yin HL, Yuan J. J Cell Biol. 2008 Apr 21. 181(2):321-33. 10.1083/jcb.200711082 PubMed 18411310