|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45060||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6755
- Total vector size (bp) 9031
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namehuman transferrin receptor
SpeciesH. sapiens (human)
Insert Size (bp)2276
Entrez GeneTFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
- Promoter Chicken Beta Actin
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TTCGGCTTCTGGCGTGTGAC
- 3′ sequencing primer GTGGTTTGTCCAAACTCATC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byKif 5c received from Caroine Enns, Oregon Health & Science University
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBa.TfR.GFP was a gift from Gary Banker & Marvin Bentley (Addgene plasmid # 45060 ; http://n2t.net/addgene:45060 ; RRID:Addgene_45060)
For your References section:The role of selective transport in neuronal protein sorting. Burack MA, Silverman MA, Banker G. Neuron. 2000 May;26(2):465-72. 10.1016/S0896-6273(00)81178-2 PubMed 10839364