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pWZL hygro H-Ras V12
(Plasmid #18749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18749 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWZL Hygro
  • Backbone manufacturer
    Available at Addgene (#18750)
  • Backbone size w/o insert (bp) 5915
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-Ras V12
  • Alt name
    HRas V12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    526
  • Mutation
    V12
  • GenBank ID
    NM_005343
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, KRAS, KRAS2, Ki-Ras, RASH1, RASK2, c-K-ras, c-Ki-ras, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jay Morgenstern
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL hygro H-Ras V12 was a gift from Scott Lowe (Addgene plasmid # 18749 ; http://n2t.net/addgene:18749 ; RRID:Addgene_18749)
  • For your References section:

    Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Cell. 1997 Mar 7. 88(5):593-602. 10.1016/S0092-8674(00)81902-9 PubMed 9054499