168,234 results

1. GFP-PKI nls

   Plasmid

   #118301

   Purpose

   Expression of PKIa

   Depositor

   Philip Stork

   Article

   Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

   Insert

   PKI nls (aa 2-36)
   

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   inserting 2xnls

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAF0286 Ef1a-Cp36_attB-BFP acceptor

   Plasmid

   #193474

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pECE-M2-PP1Cbeta

   Plasmid

   #31677

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
   

   Tags

   M2

   Expression

   Mammalian

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

4. RXFP4-Tango

   Plasmid

   #66495

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   RXFP4 (RXFP4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCS2 xenopus chordin myc

   Plasmid

   #24682

   Depositor

   Edward De Robertis

   Article

   Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

   Insert

   Chordin
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221-SV2C_STOP

   Plasmid

   #161463

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SV2C (SV2C Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGEX-4T-1 3xHA-MKK6-EE

   Plasmid

   #47579

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK6-EE (MAP2K6 Human)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCR1063

   Plasmid

   #111091

   Purpose

   Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   dCas9-KRAB
   

   Use

   Lentiviral

   Tags

   mCherry

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)

   Viral Prep

   #162381-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221-SLC17A8_STOP

    Plasmid

    #161127

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC17A8 (SLC17A8 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. mEmerald-DZIP1-N-18

    Plasmid

    #54070

    Purpose

    Localization: Centrosomes, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    DZIP1 (DZIP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pBM16

    Plasmid

    #45344

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. NHA-Ago1

    Plasmid

    #21535

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Tags

    NHA

    Expression

    Mammalian

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ITGB2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176983

    Purpose

    Mammalian expression vector encoding ITGB2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    ITGB2 (ITGB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_CSNK1E_WT

    Plasmid

    #82299

    Purpose

    Gateway Donor vector containing CSNK1E, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CSNK1E (CSNK1E Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)

    Viral Prep

    #174007-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Promoter

    Syn

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

20. pDR-AmTrac

    Plasmid

    #47770

    Purpose

    expresses AmTrac in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac
    

    Tags

    mcpGFP

    Expression

    Yeast

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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