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RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors.
Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
106219AAV CMV-dSaCas9-KRAB-bGHpAAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor
106248AAV-StufferDestination vector for U6::gRNA expression cassette
106249pLV hUbC-dSaCas9-KRAB-T2A-PuroRLentiviral vector with puro selection for expression of S. aureus dCas9-KRAB