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AAV-Stuffer
(Plasmid #106248)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106248 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTR
  • Backbone size (bp) 7000
  • Modifications to backbone
    Added stuffer sequence
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    SURE2 is also an acceptable E. coli growth strain
  • Copy number
    Unknown

Cloning Information

  • Cloning method Unknown

Resource Information

Depositor Comments

Vector used as a landing pad to clone in U6-S. aureus guide RNA expression cassette using the KpnI and XbaI sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Stuffer was a gift from Charles Gersbach (Addgene plasmid # 106248 ; http://n2t.net/addgene:106248 ; RRID:Addgene_106248)
  • For your References section:

    RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors. Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA. Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4. 10.1038/s41467-018-04048-4 PubMed 29700298