165,839 results

1. pAG148 FRB-NFAST

   Plasmid

   #130812

   Purpose

   Expresses FRB-NFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   FRB-NFAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPSU2

   Plasmid

   #89566

   Purpose

   High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders

   Depositor

   Song Tan

   Article

   Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

   Inserts

   750 bp EcoRV fragment
   3000 bp EcoRV fragment
   4000 bp EcoRV fragment
   50 bp PstI fragment
   100 bp PstI fragment
   200 bp PstI fragment
   300 bp PstI fragment
   400 bp PstI fragment
   500 bp PstI fragment
   600 bp PstI fragment
   1500 bp Psti fragment
   4100 bp PstI fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CL20_mEGFP_NUP98_KDM5A

   Plasmid

   #205872

   Purpose

   Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NUP98_KDM5A (NUP98, KDM5A Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV_DENV4_PrME

   Plasmid

   #207266

   Purpose

   Mammalian expression of Dengue virus 4 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV4_PrME (POLY Dengue virus 4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEGFP-Y705F-STAT3

   Plasmid

   #71445

   Purpose

   Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Y705F

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Ub

   Plasmid

   #11928

   Purpose

   Used for in vivo monitoring of ubiquitin localization.

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18-mini-Tn7T-Gm-lux

   Plasmid

   #64963

   Purpose

   mini-Tn7 luxCDABE transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   lux operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3.1-IGF1R-V5/HIS

   Plasmid

   #201979

   Purpose

   expression of human IGF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   IGF1R receptor tyrosine kinase (IGF1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. GW1-Peredox-mCitrine

    Plasmid

    #32386

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT_TO_FlagHA_USP10

    Plasmid

    #127102

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    USP10 (USP10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmScarlet_peroxisome_C1

    Plasmid

    #85063

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid

    #105553

    Purpose

    AAV expression of Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAL4-DBD-MCS

    Plasmid

    #145245

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-LYC

    Plasmid

    #53270

    Purpose

    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-PR rep/cap

    Plasmid

    #197565

    Purpose

    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

    Depositor

    Casey Maguire

    Article

    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

    Inserts

    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    

    Use

    AAV

    Tags

    Expression

    Mutation

    contains 7mer insert PRPPSTH between amino acids …

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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