168,234 results

1. Ubi:ccdb + RUBY

   Plasmid

   #233306

   Purpose

   GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb + RUBY
   

   Use

   Gateway destination

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-sgDHODH-2

   Plasmid

   #186023

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXint129

   Plasmid

   #178208

   Purpose

   Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

   Depositor

   Gregory Phillips

   Article

   Platt et al Plasmid. 2000 Jan . 43(1):12-23.

   Insert

   xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
   

   Expression

   Bacterial

   Promoter

   lacI

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN1-COX8-mScarletI

   Plasmid

   #129341

   Purpose

   Mitochondria matrix localization

   Depositor

   Yasushi Okada

   Insert

   mScarlet(i) (Addgene 85044)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB_smFLAG_KDM5B_MS2

   Plasmid

   #81084

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe zeo HMGA2

   Plasmid

   #17411

   Depositor

   Tyler Jacks

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

   Insert

   HMGA2 (Hmga2 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1_V5_APEX_FLAG_Ct

   Plasmid

   #194069

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-APEX-V5

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-EFNB2 (WT)

   Plasmid

   #200975

   Purpose

   Mammalian expression plasmid for wild type, myc-tagged EFNB2

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1 Bach1 S990A

    Plasmid

    #73643

    Purpose

    Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

    Depositor

    Sharon Cantor

    Insert

    Bach1 (BACH1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    S990A (BRCA binding mutant)

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pLB038 - PHS1∆P

    Plasmid

    #136285

    Purpose

    entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    PHS1∆P
    

    Expression

    Bacterial

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPOTv7-blast-3HA

    Plasmid

    #221048

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3xHA epitopes
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0943

    Plasmid

    #143246

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LMO2 (LMO2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAF0185 Cp36_attP-mCherry donor

    Plasmid

    #193468

    Purpose

    Promoterless mcherry donor for Cp36

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLexm

    Plasmid

    #99844

    Purpose

    mammalian expression

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. RXFP2-Tango

    Plasmid

    #66493

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP2 (RXFP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Tags

    2XFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-HAPLN1

    Plasmid

    #200777

    Purpose

    Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Promoter

    Synapsin

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mTORC1 Focused library

    Pooled Library

    #164084

    Purpose

    Lentiviral CRISPR library targets human mTORC1 signaling pathway

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only