|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19123||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7247
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C, grows slowly.
Copy numberHigh Copy
Gene/Insert namesmall hairpin RNA against CD44
Alt nameCDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneCD44 (a.k.a. CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer U6-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
shRNA to human CD44 (should target all isoforms). Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence
shRNA oligo sequence: sense:5'-CCGGTATGCAATGTGCTACTGATTGTCTCGAGACAATCAGTAGCACATTGCATTTTTTG-3'
Please note that the shRNA oligo present in this plasmid is referred to as shCD44-3 in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:shCD44-2 pRRL was a gift from Bob Weinberg (Addgene plasmid # 19123 ; http://n2t.net/addgene:19123 ; RRID:Addgene_19123)
For your References section:Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA. Cell. 2008 Jul 11. 134(1):62-73. 10.1016/j.cell.2008.06.006 PubMed 18614011