|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19126||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepWZL Blast
Backbone manufacturerMillenium Pharmaceuticals
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination.
Copy numberHigh Copy
Gene/Insert nameCD44 standard
Alt nameCDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
SpeciesH. sapiens (human)
Insert Size (bp)1300
Entrez GeneCD44 (a.k.a. CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBABE 5 (Common Sequencing Primers)
Human CD44 cDNA,standard form no variable exons.
CD44 protein is stably expressed in MCF7Ras or HMEC-T cells but only transiently in BPEC-T cells. Use pBabe-CD44S for BPEC-T cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CD44S pWZL-Blast was a gift from Bob Weinberg (Addgene plasmid # 19126 ; http://n2t.net/addgene:19126 ; RRID:Addgene_19126)
For your References section:Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA. Cell. 2008 Jul 11. 134(1):62-73. 10.1016/j.cell.2008.06.006 PubMed 18614011