CD44S pWZL-Blast
(Plasmid #19126)

• Depositing Lab: Bob Weinberg
• Publication: Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Backbone

• Vector backbone: pWZL Blast
• Backbone manufacturer: Millenium Pharmaceuticals
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD44 standard
• Alt name: CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: CD44 (a.k.a. CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBABE 5

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human CD44 cDNA,standard form no variable exons.
CD44 protein is stably expressed in MCF7Ras or HMEC-T cells but only transiently in BPEC-T cells. Use pBabe-CD44S for BPEC-T cells.