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pTRKH3-slpGFP
(Plasmid #27168)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27168 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    erythromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
  • Alt name
    Lactobacillus acidophilus slp promoter
  • Alt name
    synthetic construct mgfp5
  • Species
    Lactococcus lactis and synthetic construct
  • Insert Size (bp)
    1065

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AGTGAAAAGTTCTTCTCCTT
  • 3′ sequencing primer GCGGCACGACTTCTTCAAGAGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRKH3-slpGFP was a gift from Michela Lizier (Addgene plasmid # 27168 ; http://n2t.net/addgene:27168 ; RRID:Addgene_27168)
  • For your References section:

    Comparison of expression vectors in Lactobacillus reuteri strains. Lizier M, Sarra PG, Cauda R, Lucchini F. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15. 10.1111/j.1574-6968.2010.01978.x PubMed 20455948