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CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127965pMK1334sgRNA expression vector compatible with CROP-Seq and imaging screens
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127966pMTL3constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
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127967pMTL5DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
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127968pC13N-dCas9-BFP-KRABconstitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)
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127969pRT029double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
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