CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014. PubMed Article
Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127965 | pMK1334 | sgRNA expression vector compatible with CROP-Seq and imaging screens |
| 127966 | pMTL3 | constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus |
| 127967 | pMTL5 | DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus |
| 127968 | pC13N-dCas9-BFP-KRAB | constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab) |
| 127969 | pRT029 | double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus |