Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #85758)


Item Catalog # Description Quantity Price (USD)
Plasmid 85758 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Prof. Ikuko Hara-Nishimura
  • Total vector size (bp) 18518
  • Modifications to backbone
    AtU6.26p::AarIx2:sgRNA is inserted.
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin ; TagRFP in seeds

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    human-codon-optimized SpCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
  • Promoter AtRPS5A
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    human-codon-optimized SpCas9 was closed from pX330-U6-Chimeric_BB-CBh-hSpCas9 (item #42230) that we bought from Addgene on 6th June, 2013 by Prof. Tetsuya Higashiyama
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Right border (RB) may be truncated in the T-DNA integration. This may result in sgRNA scaffold truncation because the RB is close to 3' end of sgRNA. However, since we efficiently obtained knockout mutants by this vector, the truncation is not serious problem.

To overcome it, we also deposited pKI1.1R. In pKI1.1R, sgRNA is not close to RB.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKIR1.1 was a gift from Tetsuya Higashiyama (Addgene plasmid # 85758 ; ; RRID:Addgene_85758)
  • For your References section:

    pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Tsutsui H, Higashiyama T. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. 10.1093/pcp/pcw191 PubMed 27856772